Anova

#1
Hello!

I have a problem with data analysis and don't know which method to use. Here is a description of my problem: I have cells that have been treated with different conditions. Group 1 is the control group that has been treated with nothing for 12 days. Group 2 has been treated with nothing for 6 days and with Compound X for another 6 days. Group 3 has been treated with Compound Y for 6 days and then with Compound X for another 6 days. Group 4 has been treated with Compound Y for 12 days. At the end of 12 days, the expression of gene Z is measured. I want to show that the gene expression level is different between these groups. Do I need to do one-way or two-way ANOVA? Should I consider compounds X and Y as one variable or as two? Also is there an issue with the difference in treatment duration? Please help!!

Thank you!