For my thesis I'm doing a factorial Mixed ANOVA in which I look at gender differences. As it's a mixed ANOVA, everyone goes through the same conditions. The problem however is that I have 10 men and 26 women in my sample. Therefore the groups that I compare when looking at gender, aren't equal. I was wondering if there was a specific test I could do to calculate how problematic this difference in gender ratio is and if there is a test I could do to make up for this discrepancy?

Thank a lot in advance.